Seeking the sweet spot to beat a pig parasite
Outbreaks of the nematode , also known as porcine whipworm, can be costly for pig farmers and inflict suffering on animals with severe infections. This worm reproduces in pigs’ digestive tracts, causing dehydration, malnourishment and sometimes death. Understanding how a pig's immune system reacts to T. suis is an important step toward developing better strategies for helping animals to clear it.
Iain B.H. Wilson’s lab at the Universit盲t fu虉r Bodenkultur in Vienna, in collaboration with Richard Cummings’ lab at Harvard Medical School, tackled the question of how the pig immune system recognizes sugar molecules, or glycans, found on T. suis proteins. The group recently reported in the journal 麻豆传媒色情片 & Cellular Proteomics. Lead author Barbara Eckmair, along with co-author Katharina Paschinger and Wilson walked us through their process, their findings and the importance of this study.
The group extracted glycans from worms and then separated different types of glycans using high-performance liquid chromatography, a technique that isolates compounds within a mixture based on how easily they separate due to their size and structure. They then created arrays of spots made up of the separated glycans. Using these arrays, they tested several innate immune system proteins, as well as immunoglobulin G and M, or IgG and IgM, antibodiesfrom T. suis–infected pigs, to see how well they bound to each glycan spot.
“Some large molecular-weight glycans seemed to have the bulk of the response to the antisera,” Wilson said. “So that left the question as to what the exact structures were.”
They found that many fucose residues were common, as well as a zwitterion called phosphorylcholine, and up to eight phosphorylcholine residues could decorate some of the glycans.
“Lots of other nematodes have phosphorylcholine on their N-glycans,” he said, “and these are very often associated with immune system downregulation.”
However, this was the first time such large structures had been found in T. suis.
Wilson said this research is especially impactful for organic farming because animals raised this way receive fewer antiparasitic drugs and are at higher risk of worm infections. suggest that vaccination against these parasites is an attractive potential strategy.
“There are indications from Haemonchus, which is a sheep parasite, that the glycans would be important for making the vaccine," Wilson said.
When asked about the next steps for this research, co-author Paschinger said that determining which glycans are bound by IgE, an antibody that plays a key role in the immune response to parasites, is an important question to answer.
Eckmair, the paper’s lead author, explained that IgE binding could not be addressed in this study because the reagents needed to test pig IgE binding are difficult to procure, but she said future experiments could tackle this and many other questions.
“Of course, there is more immunological work that we could do, because we still have some of the arrays left,” Eckmair said. “This was really a lucky case because we have so much material that we could test.”
This research is not only a step toward understanding the pig immune response to T. suis; by helping researchers understand the immune response to glycans found in the human whipworm, it might lead to medical advances for humans as well.
Enjoy reading ASBMB Today?
Become a member to receive the print edition monthly and the digital edition weekly.
Learn moreGet the latest from ASBMB Today
Enter your email address, and we鈥檒l send you a weekly email with recent articles, interviews and more.
Latest in Science
Science highlights or most popular articles
Can a hair-loss drug prevent heart disease?
With the approved medication finasteride, researchers in Illinois may have found a new way to kill two birds with one stone.
These proteins have been secretly managing your cells
Scientists have long known that histones spool DNA and help regulate genes. They may be doing a lot more.
At the Salton Sea, uncovering the culprit of lung disease
Scientists have long suspected a link between the dust and poor respiratory health. According to recent findings, the prime suspect is a naturally occurring toxin.
From the journals: MCP
The importance of sharing proteomics data. Detecting nitrotyrosine-containing proteins. Analyzing yeast proteasomes. Read about these recent articles.
Using a network to snare the cause of kidney disease
A microfluidic device that mimics blood capillaries may help in early detection, and proper measures could reduce the risk of renal failures.
All about cholesterol
The latest science on how blood levels of HDL, LDL and more relate to cardiovascular health.