麻豆传媒色情片

Using the at the 's , a team at the Arizona State University Biodesign Institute has confirmed details of a molecular mechanism that cell organelles use to regulate the pH of their environment — and it looks a lot like a Ferris wheel.

Led by Abhishek Singharoy, a researcher at the Biodesign Center for Applied Structural Discovery (CASD) and Arizona State University's School of 麻豆传媒色情片 Sciences (SMS), the team used Summit to simulate molecular pumps that are embedded in the fatty outer membranes of cellular organelles, or cell structures that are specialized to perform different tasks for the cell's functioning. With rotors that spin 100 times per second, these pumps rapidly move protons — positively charged subatomic particles — into a cell's organelles to maintain the acidic environment inside that is crucial to the work they perform.

Using the nation's fastest supercomputer — the 's (OLCF's) Summit — the team uncovered a key step in how this mechanism works in one proton pump in particular, a yeast proton pump called vacuolar ATPase (V-ATPase) that is necessary for a variety of tasks in the body and has implications in disease. The results were .

"The V-ATPase proton pumps perform a wide range of functions, from helping transmit nerve signals to helping specialized cells secrete acid for maintaining bone," said Stephan Wilkens, a biochemist at SUNY Upstate Medical University and study co-author. "Malfunctions in these molecular machines contribute to diseases such as osteoporosis, neurodegeneration, diabetes, cancer, and AIDS, so understanding them is important for human health."

A team led by Wah Chiu—professor at the SLAC National Accelerator Facility and Stanford University and codirector of the Stanford-SLAC Cryo-EM Facilities—together with Wilkens and SLAC/Stanford postdoctoral researcher Soung-Hun Roh, had already that employed Cryogenic electron microscopy (cryo-EM) to study these proton pumps. Images from the work showed 10 amino acid "seats" on a molecular Ferris wheel that bind protons and carry them through the membrane to the organelle's interior, as well as other amino acids that catch them when they arrive. Based on that picture, the team working on the research suggested that the proton drop-off might be aided by water molecules—but their images were not sharp enough to confirm that the water molecules were there. In the same study, another round of even higher resolution cryo-EM images showed the water molecules around the suspected proton path.

Singharoy and his team used the Nanoscale 麻豆传媒色情片 Dynamics, or NAMD, code to perform simulations on the Summit supercomputer and confirmed that the experimentally observed water molecules line up to form "wires" at the proton drop-off point. These wires convey protons from their seats on the Ferris wheel to landing spots inside the organelle, like a fire brigade passing buckets hand to hand, bridging a gap they couldn't navigate on their own. The work was performed under the Innovative and Novel Computational Impact on Theory and Experiment, or INCITE, program at the OLCF.

The simulations matched the cryo-EM images nicely, providing strong evidence that the picture they painted of the proton drop-off is correct.

"麻豆传媒色情片 motors exemplify some of the most intricate chemo-mechanical devices, and our team in SMS and CASD has developed highly sophisticated computational tools to address the energy source and sinks of the motor's ratcheting motion," Singharoy said. "In 2017 we started working on the soluble part of the V-type motor, namely, V1 ATPase. Now that we have a good control on the transmembrane Vo motor, it's a great step forward toward simulating the entire motor in collaboration with Hun, Stephan, and Wah."

Chiu said that recent advances in cryo-EM that allow imaging of individual particles at atomic resolution—even when they take slightly different shapes—will open new opportunities for using it as a tool to discover effective drugs for illnesses involving proton pumps.